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Biosensing, Imaging, and Microscopy |
Distinguishing between whole cells and cell debris
Distinguishing between whole cells and cell debris is important in microscopy, e.g., in screening of pulmonary patients for infectious tuberculosis. We propose and theoretically demonstrate that whole cells and cell debris can be distinguished from the far-field pattern of surface plasmon coupled emission (SPCE) of a fluorescently-labeled sample placed on a thin metal layer. If fluorescently-labeled whole cells are placed on the metal film, SPCE takes place simultaneously at two or more different angles and creates two or more distinct rings in the far field. By contrast, if fluorescently-labeled cell debris are placed on the metal film, SPCE takes place at only one angle and creates one ring in the far-field. We find that the angular separation of the far-field rings is sufficiently distinct to use the presence of one or more rings to distinguish between whole cells and cell debris. The proposed technique has the potential for detection without the use of a microscope. |
Publication:
M. A. Talukder, C. R. Menyuk, and Y. Kostov, "Distinguishing between whole cells and cell debris using surface plasmon coupled emission," Biomedical Optics Express 9, 1977 (2018). [ PDF ]
A. Das and M. A. Talukder, "Theoretical analysis of bimetallic nanorod dimer biosensors for label-free molecule detection," AIP Advances 8, 025302 (2018). [ PDF ]
S. Z. Uddin and M. A. Talukder, "Imaging of cell membrane topography using Tamm plasmon coupled emission," Biomedical Physics and Engineering Express 3, 065005 (2017). [ PDF ]
S. Z. Uddin and M. A. Talukder, "Two-dimensional materials for improved resolution in total internal reflection fluorescence microscopy," Materials Research Express 4, 096203 (2017). [ PDF ]
S. Z. Uddin, M. R. Tanvir, and M. A. Talukder, "A proposal and an analysis of an enhanced surface plasmon coupled emission structure for single molecule detection," J. Appl. Phys. 119, 204701 (2016). [ PDF ]
M. A. Talukder, C. R. Menyuk, and Y. Kostov, "Distinguishing between whole cells and cell debris using surface plasmon coupled emission," Photonics West, San Francisco, CA, USA (2017).
S. Z. Uddin and M. A. Talukder, "Reduction of detection volume in total internal reflection fluorescence microscopy using graphene," 9th International Conference on Electrical and Computer Engineering (ICECE), Dhaka, Bangladesh (2016).
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| Principal Investigator |
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Dr. Muhammad Anisuzzaman Talukder
Office : ECE 527
Dept. of EEE
BUET
Dhaka 1000
Phone 9665650 Ext. 6582
FAX 8613046 |
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| News & Updates |
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Apr 26, 2019
Dr. Anis gave an invited talk at ICEECC 2019. more
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Apr 9, 2019
Paper accepted in Plasmonics. more
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Jan 15, 2019
Article published in Biggan Chinta. more
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Dec 24, 2018
Dr. Anis gave an invited talk at National Public Library. more
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